Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26038153
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Crit+Rev+Biochem+Mol+Biol
2015 ; 50
(4
): 269-83
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Protein-DNA binding in high-resolution
#MMPMID26038153
Mahony S
; Pugh BF
Crit Rev Biochem Mol Biol
2015[]; 50
(4
): 269-83
PMID26038153
show ga
Recent advances in experimental and computational methodologies are enabling
ultra-high resolution genome-wide profiles of protein-DNA binding events. For
example, the ChIP-exo protocol precisely characterizes protein-DNA cross-linking
patterns by combining chromatin immunoprecipitation (ChIP) with 5' ? 3'
exonuclease digestion. Similarly, deeply sequenced chromatin accessibility assays
(e.g. DNase-seq and ATAC-seq) enable the detection of protected footprints at
protein-DNA binding sites. With these techniques and others, we have the
potential to characterize the individual nucleotides that interact with
transcription factors, nucleosomes, RNA polymerases and other regulatory proteins
in a particular cellular context. In this review, we explain the experimental
assays and computational analysis methods that enable high-resolution profiling
of protein-DNA binding events. We discuss the challenges and opportunities
associated with such approaches.
free
free
free
