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2016 ; 580
(ä): 45-87
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Protein and Antibody Engineering by Phage Display
#MMPMID27586328
Frei JC
; Lai JR
Methods Enzymol
2016[]; 580
(ä): 45-87
PMID27586328
show ga
Phage display is an in vitro selection technique that allows for the rapid
isolation of proteins with desired properties including increased affinity,
specificity, stability, and new enzymatic activity. The power of phage display
relies on the phenotype-to-genotype linkage of the protein of interest displayed
on the phage surface with the encoding DNA packaged within the phage particle,
which allows for selective enrichment of library pools and high-throughput
screening of resulting clones. As an in vitro method, the conditions of the
binding selection can be tightly controlled. Due to the high-throughput nature,
rapidity, and ease of use, phage display is an excellent technological platform
for engineering antibody or proteins with enhanced properties. Here, we describe
methods for synthesis, selection, and screening of phage libraries with
particular emphasis on designing humanizing antibody libraries and combinatorial
scanning mutagenesis libraries. We conclude with a brief section on
troubleshooting for all stages of the phage display process.
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