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2016 ; 15
(12
): 2625-36
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Protein Abundance Control by Non-coding Antisense Transcription
#MMPMID27292640
Huber F
; Bunina D
; Gupta I
; Khmelinskii A
; Meurer M
; Theer P
; Steinmetz LM
; Knop M
Cell Rep
2016[Jun]; 15
(12
): 2625-36
PMID27292640
show ga
Stable unannotated transcripts (SUTs), some of which overlap protein-coding genes
in antisense direction, are a class of non-coding RNAs. While case studies have
reported important regulatory roles for several of such RNAs, their general
impact on protein abundance regulation of the overlapping gene is not known. To
test this, we employed seamless gene manipulation to repress antisense SUTs of
162 yeast genes by using a unidirectional transcriptional terminator and a GFP
tag. We found that the mere presence of antisense SUTs was not sufficient to
influence protein abundance, that observed effects of antisense SUTs correlated
with sense transcript start site overlap, and that the effects were generally
weak and led to reduced protein levels. Antisense regulated genes showed
increased H3K4 di- and trimethylation and had slightly lower than expected noise
levels. Our results suggest that the functionality of antisense RNAs has gene and
condition-specific components.
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