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10.1186/s12934-015-0344-z http://scihub22266oqcxt.onion/10.1186/s12934-015-0344-z C4595204!4595204 !26438232     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26438232 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Microb+Cell+Fact 2015 ; 14 (ä): 154 Nephropedia Template TP {{tp|p=26438232 |t=2015. Prophage recombinases-mediated genome engineering in Lactobacillus plantarum |pdf=|usr=}}{{26438232 }} gab.com Text Prophage recombinases-mediated genome engineering in Lactobacillus plantarum JO: Microb Cell Fact http://www.kidney.de/pget.php?&tw=1&pmid=26438232 #FOAvip BACKGROUND: Lactobacillus plantarum is a food-grade microorganism with industrial and medical relevance belonging to the group of lactic acid bacteria (LAB). Traditional strategies for obtaining gene deletion variants in this organism are mainly vector-based double-crossover methods, which are inefficient and laborious. A feasible possibility to solve this problem is the recombineering, which greatly expands the possibilities for engineering DNA molecules in vivo in various organisms. RESULTS: In this work, a double-stranded DNA (dsDNA) recombineering system was established in L. plantarum. An exonuclease encoded by lp_0642 and a potential host-nuclease inhibitor encoded by lp_0640 involved in dsDNA recombination were identified from a prophage P1 locus in L. plantarum WCFS1. These two proteins, combined with the previously characterized single strand annealing protein encoded by lp_0641, can perform homologous recombination between a heterologous dsDNA substrate and host genomic DNA. Based on this, we developed a method for marker-free genetic manipulation of the chromosome in L. plantarum. CONCLUSIONS: This Lp_0640-41-42-mediated recombination allowed easy screening of mutants and could serve as an alternative to other genetic manipulation methods. We expect that this method can help for understanding the probiotic functionality and physiology of LAB. Twit Text FOAVip Prophage recombinases-mediated genome engineering in Lactobacillus plantarum ????Microb Cell Fact http://www.kidney.de/pget.php?&tw=1&pmid=26438232 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26438232 #FOAvip English Wikipedia <ref>{{Cite pmid|26438232 }}</ref> Prophage recombinases-mediated genome engineering in Lactobacillus plantarum #MMPMID26438232 Yang P ; Wang J ; Qi Q Microb Cell Fact 2015[Oct]; 14 (ä): 154 PMID26438232 show ga BACKGROUND: Lactobacillus plantarum is a food-grade microorganism with industrial and medical relevance belonging to the group of lactic acid bacteria (LAB). Traditional strategies for obtaining gene deletion variants in this organism are mainly vector-based double-crossover methods, which are inefficient and laborious. A feasible possibility to solve this problem is the recombineering, which greatly expands the possibilities for engineering DNA molecules in vivo in various organisms. RESULTS: In this work, a double-stranded DNA (dsDNA) recombineering system was established in L. plantarum. An exonuclease encoded by lp_0642 and a potential host-nuclease inhibitor encoded by lp_0640 involved in dsDNA recombination were identified from a prophage P1 locus in L. plantarum WCFS1. These two proteins, combined with the previously characterized single strand annealing protein encoded by lp_0641, can perform homologous recombination between a heterologous dsDNA substrate and host genomic DNA. Based on this, we developed a method for marker-free genetic manipulation of the chromosome in L. plantarum. CONCLUSIONS: This Lp_0640-41-42-mediated recombination allowed easy screening of mutants and could serve as an alternative to other genetic manipulation methods. We expect that this method can help for understanding the probiotic functionality and physiology of LAB. |*Genome, Bacterial [MESH] |DNA/genetics/metabolism [MESH] |Escherichia coli Proteins/genetics [MESH] |Escherichia coli/enzymology [MESH] |Genetic Loci [MESH] |Genetic Vectors/genetics/metabolism [MESH] |Glucuronidase/genetics [MESH] |Homologous Recombination [MESH] |Lactobacillus plantarum/*genetics/virology [MESH] |Prophages/*enzymology [MESH] |Recombinases/*genetics/metabolism [MESH]Prophage recombinases-mediated genome engineering in Lactobacillus pla(epmc).pdf DeepDyve Pubget Overpricing