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2015 ; 40
(1
): 78-82
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Prohibitin as a novel autoantigen in rheumatoid arthritis
#MMPMID26155187
Shi LL
; Chen P
; Xun YP
; Yang WK
; Yang CH
; Chen GY
; Du HW
Cent Eur J Immunol
2015[]; 40
(1
): 78-82
PMID26155187
show ga
THE AIM OF THIS STUDY: The aim of this study was to verify whether prohibitin is
a novel autoantigen in rheumatoid arthritis. MATERIAL AND METHODS: First,
recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified.
Then the anti-prohibitin autoantibodies were detected by western blotting by
using rhPHB protein to incubate sera from patients with rheumatoid arthritis
(RA). Next, immunoprecipitation was employed to further illustrate whether
anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies
against the rhPHB protein were investigated using a homemade ELISA kit through
the assessment of 258 real clinical samples. RESULTS: It was revealed that
anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of
serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86
systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently
healthy donors (HC) (1.2%). CONCLUSIONS: Prohibitin was proved to be a novel
autoantigen and the corresponding anti-prohibitin autoantibodies were present in
the RA patients' blood circulation.