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2017 ; 7
(3
): 789-804
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Progress in Exosome Isolation Techniques
#MMPMID28255367
Li P
; Kaslan M
; Lee SH
; Yao J
; Gao Z
Theranostics
2017[]; 7
(3
): 789-804
PMID28255367
show ga
Exosomes are one type of membrane vesicles secreted into extracellular space by
most types of cells. In addition to performing many biological functions
particularly in cell-cell communication, cumulative evidence has suggested that
several biological entities in exosomes like proteins and microRNAs are closely
associated with the pathogenesis of most human malignancies and they may serve as
invaluable biomarkers for disease diagnosis, prognosis, and therapy. This
provides a commanding impetus and growing demands for simple, efficient, and
affordable techniques to isolate exosomes. Capitalizing on the physicochemical
and biochemical properties of exosomes, a number of techniques have been
developed for the isolation of exosomes. This article summarizes the advances in
exosome isolation techniques with an emphasis on their isolation mechanism,
performance, challenges, and prospects. We hope that this article will provide an
overview of exosome isolation techniques, opening up new perspectives towards the
development more innovative strategies and devices for more time saving, cost
effective, and efficient isolations of exosomes from a wide range of biological
matrices.