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10.1038/nchem.2872 http://scihub22266oqcxt.onion/10.1038/nchem.2872 C5784857!5784857 !29359755     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29359755 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Chem 2018 ; 10 (2 ): 155-164 Nephropedia Template TP {{tp|p=29359755 |t=2018. Programmable autonomous synthesis of single-stranded DNA |pdf=|usr=}}{{29359755 }} gab.com Text Programmable autonomous synthesis of single-stranded DNA JO: Nat Chem http://www.kidney.de/pget.php?&tw=1&pmid=29359755 #FOAvip DNA performs diverse functional roles in biology, nanotechnology and biotechnology, but current methods for autonomously synthesizing arbitrary single-stranded DNA are limited. Here, we introduce the concept of primer exchange reaction (PER) cascades, which grow nascent single-stranded DNA with user-specified sequences following prescribed reaction pathways. PER synthesis happens in a programmable, autonomous, in situ and environmentally responsive fashion, providing a platform for engineering molecular circuits and devices with a wide range of sensing, monitoring, recording, signal-processing and actuation capabilities. We experimentally demonstrate a nanodevice that transduces the detection of a trigger RNA into the production of a DNAzyme that degrades an independent RNA substrate, a signal amplifier that conditionally synthesizes long fluorescent strands only in the presence of a particular RNA signal, molecular computing circuits that evaluate logic (AND, OR, NOT) combinations of RNA inputs, and a temporal molecular event recorder that records in the PER transcript the order in which distinct RNA inputs are sequentially detected. Twit Text FOAVip Programmable autonomous synthesis of single-stranded DNA ????Nat Chem http://www.kidney.de/pget.php?&tw=1&pmid=29359755 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29359755 #FOAvip English Wikipedia <ref>{{Cite pmid|29359755 }}</ref> Programmable autonomous synthesis of single-stranded DNA #MMPMID29359755 Kishi JY ; Schaus TE ; Gopalkrishnan N ; Xuan F ; Yin P Nat Chem 2018[Feb]; 10 (2 ): 155-164 PMID29359755 show ga DNA performs diverse functional roles in biology, nanotechnology and biotechnology, but current methods for autonomously synthesizing arbitrary single-stranded DNA are limited. Here, we introduce the concept of primer exchange reaction (PER) cascades, which grow nascent single-stranded DNA with user-specified sequences following prescribed reaction pathways. PER synthesis happens in a programmable, autonomous, in situ and environmentally responsive fashion, providing a platform for engineering molecular circuits and devices with a wide range of sensing, monitoring, recording, signal-processing and actuation capabilities. We experimentally demonstrate a nanodevice that transduces the detection of a trigger RNA into the production of a DNAzyme that degrades an independent RNA substrate, a signal amplifier that conditionally synthesizes long fluorescent strands only in the presence of a particular RNA signal, molecular computing circuits that evaluate logic (AND, OR, NOT) combinations of RNA inputs, and a temporal molecular event recorder that records in the PER transcript the order in which distinct RNA inputs are sequentially detected. |DNA, Catalytic/chemistry/*metabolism [MESH]Programmable autonomous synthesis of single-stranded DNA (epmc).pdf DeepDyve Pubget Overpricing