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10.1016/j.cell.2016.02.054 http://scihub22266oqcxt.onion/10.1016/j.cell.2016.02.054 C4826288!4826288 !26997482     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26997482 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell 2016 ; 165 (2 ): 488-96 Nephropedia Template TP {{tp|p=26997482 |t=2016. Programmable RNA Tracking in Live Cells with CRISPR/Cas9 |pdf=|usr=}}{{26997482 }} gab.com Text Programmable RNA Tracking in Live Cells with CRISPR/Cas9 JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=26997482 #FOAvip RNA-programmed genome editing using CRISPR/Cas9 from Streptococcus pyogenes has enabled rapid and accessible alteration of specific genomic loci in many organisms. A flexible means to target RNA would allow alteration and imaging of endogenous RNA transcripts analogous to CRISPR/Cas-based genomic tools, but most RNA targeting methods rely on incorporation of exogenous tags. Here, we demonstrate that nuclease-inactive S. pyogenes CRISPR/Cas9 can bind RNA in a nucleic-acid-programmed manner and allow endogenous RNA tracking in living cells. We show that nuclear-localized RNA-targeting Cas9 (RCas9) is exported to the cytoplasm only in the presence of sgRNAs targeting mRNA and observe accumulation of ACTB, CCNA2, and TFRC mRNAs in RNA granules that correlate with fluorescence in situ hybridization. We also demonstrate time-resolved measurements of ACTB mRNA trafficking to stress granules. Our results establish RCas9 as a means to track RNA in living cells in a programmable manner without genetically encoded tags. Twit Text FOAVip Programmable RNA Tracking in Live Cells with CRISPR/Cas9 ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=26997482 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26997482 #FOAvip English Wikipedia <ref>{{Cite pmid|26997482 }}</ref> Programmable RNA Tracking in Live Cells with CRISPR/Cas9 #MMPMID26997482 Nelles DA ; Fang MY ; O'Connell MR ; Xu JL ; Markmiller SJ ; Doudna JA ; Yeo GW Cell 2016[Apr]; 165 (2 ): 488-96 PMID26997482 show ga RNA-programmed genome editing using CRISPR/Cas9 from Streptococcus pyogenes has enabled rapid and accessible alteration of specific genomic loci in many organisms. A flexible means to target RNA would allow alteration and imaging of endogenous RNA transcripts analogous to CRISPR/Cas-based genomic tools, but most RNA targeting methods rely on incorporation of exogenous tags. Here, we demonstrate that nuclease-inactive S. pyogenes CRISPR/Cas9 can bind RNA in a nucleic-acid-programmed manner and allow endogenous RNA tracking in living cells. We show that nuclear-localized RNA-targeting Cas9 (RCas9) is exported to the cytoplasm only in the presence of sgRNAs targeting mRNA and observe accumulation of ACTB, CCNA2, and TFRC mRNAs in RNA granules that correlate with fluorescence in situ hybridization. We also demonstrate time-resolved measurements of ACTB mRNA trafficking to stress granules. Our results establish RCas9 as a means to track RNA in living cells in a programmable manner without genetically encoded tags. |CRISPR-Cas Systems [MESH] |Cytoplasmic Granules/chemistry [MESH] |Endonucleases/metabolism [MESH] |Glyceraldehyde-3-Phosphate Dehydrogenases/genetics [MESH] |Green Fluorescent Proteins/analysis [MESH] |Humans [MESH] |RNA, Guide, CRISPR-Cas Systems/analysis [MESH] |RNA, Messenger/analysis [MESH]Programmable RNA Tracking in Live Cells with CRISPR/Cas9 (epmc).pdf DeepDyve Pubget Overpricing