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2017 ; 4
(ä): 45
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Probing Long Non-coding RNA-Protein Interactions
#MMPMID28744458
Barra J
; Leucci E
Front Mol Biosci
2017[]; 4
(ä): 45
PMID28744458
show ga
Non-coding RNA sequences outnumber the protein-coding genes in the human genome,
however our knowledge of their functions is still limited. RNA-binding proteins
follow the transcripts, including non-coding RNAs, throughout their life,
regulating not only maturation, nuclear export, stability and eventually
translation, but also RNA functions. Therefore, development of sophisticated
methods to study RNA-protein interactions are key to the systematic
characterization of lncRNAs. Although mostly applicable to RNA-protein
interactions in general, many approaches, especially the computational ones, need
adjustment to be adapted to the length and complexity of lncRNA transcripts. Here
we critically review all the wet lab and computational methods to study
lncRNA-protein interactions and their potential to clarify the dark side of the
genome.