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2015 ; 1189
(ä): 283-97
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Primary cell cultures of regenerating holothurian tissues
#MMPMID25245701
Bello SA
; Abreu-Irizarry RJ
; García-Arrarás JE
Methods Mol Biol
2015[]; 1189
(ä): 283-97
PMID25245701
show ga
The ability to culture different cell types is essential for answering many
questions in developmental and regenerative biology. Studies in marine organisms,
in particular echinoderms, have been limited by the lack of well-described
cellular culture systems. Here we describe a cell culture system, for normal or
regenerating holothurian cells, that allows cell characterization by
immunohistochemistry and scanning electron microscopy. These cell cultures can
now be used to perform multiple types of experiments in order to explore the
cellular, biochemical, and genomic aspects of echinoderm regenerative properties.