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10.1007/978-1-4939-1164-6_19

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suck abstract from ncbi


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pmid25245701
      Methods+Mol+Biol 2015 ; 1189 (ä): 283-97
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  • Primary cell cultures of regenerating holothurian tissues #MMPMID25245701
  • Bello SA ; Abreu-Irizarry RJ ; García-Arrarás JE
  • Methods Mol Biol 2015[]; 1189 (ä): 283-97 PMID25245701 show ga
  • The ability to culture different cell types is essential for answering many questions in developmental and regenerative biology. Studies in marine organisms, in particular echinoderms, have been limited by the lack of well-described cellular culture systems. Here we describe a cell culture system, for normal or regenerating holothurian cells, that allows cell characterization by immunohistochemistry and scanning electron microscopy. These cell cultures can now be used to perform multiple types of experiments in order to explore the cellular, biochemical, and genomic aspects of echinoderm regenerative properties.
  • |Animals [MESH]
  • |Cell Shape [MESH]
  • |Cells, Cultured [MESH]
  • |Digestive System/cytology [MESH]
  • |Disinfection [MESH]
  • |Dissection [MESH]
  • |Holothuria/*cytology/ultrastructure [MESH]
  • |Immunohistochemistry [MESH]
  • |Primary Cell Culture/*methods [MESH]


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