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10.1107/S2053230X15008304

http://scihub22266oqcxt.onion/10.1107/S2053230X15008304
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C4461322!4461322 !26057787
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suck abstract from ncbi


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pmid26057787
      Acta+Crystallogr+F+Struct+Biol+Commun 2015 ; 71 (Pt 6 ): 622-42
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  • Practical macromolecular cryocrystallography #MMPMID26057787
  • Pflugrath JW
  • Acta Crystallogr F Struct Biol Commun 2015[Jun]; 71 (Pt 6 ): 622-42 PMID26057787 show ga
  • Cryocrystallography is an indispensable technique that is routinely used for single-crystal X-ray diffraction data collection at temperatures near 100 K, where radiation damage is mitigated. Modern procedures and tools to cryoprotect and rapidly cool macromolecular crystals with a significant solvent fraction to below the glass-transition phase of water are reviewed. Reagents and methods to help prevent the stresses that damage crystals when flash-cooling are described. A method of using isopentane to assess whether cryogenic temperatures have been preserved when dismounting screened crystals is also presented.
  • |Cryoprotective Agents/*chemistry [MESH]
  • |Crystallization [MESH]
  • |Crystallography, X-Ray/history/instrumentation/*methods [MESH]
  • |Freezing [MESH]
  • |History, 20th Century [MESH]
  • |History, 21st Century [MESH]
  • |Humans [MESH]
  • |Proteins/*ultrastructure [MESH]
  • |Scattering, Small Angle [MESH]
  • |Water/*chemistry [MESH]


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