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2015 ; 8
(7
): 8663-70
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Podoplanin increases migration and angiogenesis in malignant glioma
#MMPMID26339454
Grau SJ
; Trillsch F
; Tonn JC
; Goldbrunner RH
; Noessner E
; Nelson PJ
; von Luettichau I
Int J Clin Exp Pathol
2015[]; 8
(7
): 8663-70
PMID26339454
show ga
Expression of podoplanin in glial brain tumors is grade dependent. While serving
as a marker for tumor progression and modulating invasion in various neoplasms,
little is known about podoplanin function in gliomas. Therefore we stably
transfected two human glioma cell lines (U373MG and U87MG) with expression
plasmids encoding podoplanin. The efficacy of transfection was confirmed by FACS
analysis, PCR and immunocytochemistry. Cells were then sorted for highly
podoplanin expressing cells (U373P(high)/U87P(high)). Transfection did not
influence the production of pro-angiogenic factors including VEGF, VEGF-C and D.
Also, expression of VEGF receptors (VEGFR) remained unchanged except for
U87P(high), where a VEGFR3 expression was induced. U373P(high) showed
significantly reduced proliferation as compared to mock transfected group. By
contrast, podoplanin significantly increased migration and invasion into collagen
matrix. Furthermore, conditioned media from P(high) glioma cells strongly induced
tube formation on matrigel. In conclusion, podoplanin increased migration of
tumor cells and enhanced tube formation activity in endothelial cells independent
from VEGF. Thus, podoplanin expression may be an important step in tumor
progression.
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