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2018 ; 19
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Platforms for Single-Cell Collection and Analysis
#MMPMID29534489
Valihrach L
; Androvic P
; Kubista M
Int J Mol Sci
2018[Mar]; 19
(3
): ä PMID29534489
show ga
Single-cell analysis has become an established method to study cell heterogeneity
and for rare cell characterization. Despite the high cost and technical
constraints, applications are increasing every year in all fields of biology.
Following the trend, there is a tremendous development of tools for single-cell
analysis, especially in the RNA sequencing field. Every improvement increases
sensitivity and throughput. Collecting a large amount of data also stimulates the
development of new approaches for bioinformatic analysis and interpretation.
However, the essential requirement for any analysis is the collection of single
cells of high quality. The single-cell isolation must be fast, effective, and
gentle to maintain the native expression profiles. Classical methods for
single-cell isolation are micromanipulation, microdissection, and
fluorescence-activated cell sorting (FACS). In the last decade several new and
highly efficient approaches have been developed, which not just supplement but
may fully replace the traditional ones. These new techniques are based on
microfluidic chips, droplets, micro-well plates, and automatic collection of
cells using capillaries, magnets, an electric field, or a punching probe. In this
review we summarize the current methods and developments in this field. We
discuss the advantages of the different commercially available platforms and
their applicability, and also provide remarks on future developments.
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