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10.1038/s41598-017-02395-8 http://scihub22266oqcxt.onion/10.1038/s41598-017-02395-8 C5437072!5437072 !28522808     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28522808 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2017 ; 7 (1 ): 2124 Nephropedia Template TP {{tp|p=28522808 |t=2017. Plasmonics Enhanced Smartphone Fluorescence Microscopy |pdf=|usr=}}{{28522808 }} gab.com Text Plasmonics Enhanced Smartphone Fluorescence Microscopy JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28522808 #FOAvip Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50?nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings. Twit Text FOAVip Plasmonics Enhanced Smartphone Fluorescence Microscopy ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28522808 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28522808 #FOAvip English Wikipedia <ref>{{Cite pmid|28522808 }}</ref> Plasmonics Enhanced Smartphone Fluorescence Microscopy #MMPMID28522808 Wei Q ; Acuna G ; Kim S ; Vietz C ; Tseng D ; Chae J ; Shir D ; Luo W ; Tinnefeld P ; Ozcan A Sci Rep 2017[May]; 7 (1 ): 2124 PMID28522808 show ga Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50?nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings. |*Smartphone [MESH] |Glass/chemistry [MESH] |Image Enhancement/instrumentation/*methods [MESH] |Microscopy, Fluorescence/instrumentation/methods [MESH] |Point-of-Care Testing [MESH] |Quantum Dots/*chemistry [MESH]Plasmonics Enhanced Smartphone Fluorescence Microscopy (epmc).pdf DeepDyve Pubget Overpricing