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2017 ; 8
(11
): 5040-5048
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Photodynamic opening of blood-brain barrier
#MMPMID29188101
Semyachkina-Glushkovskaya O
; Kurths J
; Borisova E
; Sokolovski S
; Mantareva V
; Angelov I
; Shirokov A
; Navolokin N
; Shushunova N
; Khorovodov A
; Ulanova M
; Sagatova M
; Agranivich I
; Sindeeva O
; Gekalyuk A
; Bodrova A
; Rafailov E
Biomed Opt Express
2017[Nov]; 8
(11
): 5040-5048
PMID29188101
show ga
Photodynamic treatment (PDT) causes a significant increase in the permeability of
the blood-brain barrier (BBB) in healthy mice. Using different doses of laser
radiation (635 nm, 10-40 J/cm(2)) and photosensitizer (5-aminolevulinic acid -
5-ALA, 20 and 80 mg/kg, i.v.), we found that the optimal PDT for the reversible
opening of the BBB is 15 J/cm(2) and 5-ALA, 20 mg/kg, exhibiting brain tissues
recovery 3 days after PDT. Further increases in the laser radiation or 5-ALA
doses have no amplifying effect on the BBB permeability, but are associated with
severe damage of brain tissues. These results can be an informative platform for
further studies of new strategies in brain drug delivery and for better
understanding of mechanisms underlying cerebrovascular effects of PDT-related
fluorescence guided resection of brain tumor.