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10.1016/j.cell.2014.10.004 http://scihub22266oqcxt.onion/10.1016/j.cell.2014.10.004 C4243060!4243060 !25417167     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25417167 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25417167 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell 2014 ; 159 (4 ): 940-54 Nephropedia Template TP {{tp|p=25417167 |t=2014. Paper-based synthetic gene networks |pdf=|usr=}}{{25417167 }} gab.com Text Paper-based synthetic gene networks JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=25417167 #FOAvip Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid prototyping of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors. Twit Text FOAVip Paper-based synthetic gene networks ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=25417167 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25417167 #FOAvip English Wikipedia <ref>{{Cite pmid|25417167 }}</ref> Paper-based synthetic gene networks #MMPMID25417167 Pardee K ; Green AA ; Ferrante T ; Cameron DE ; DaleyKeyser A ; Yin P ; Collins JJ Cell 2014[Nov]; 159 (4 ): 940-54 PMID25417167 show ga Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid prototyping of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors. |*Cell-Free System [MESH] |*Gene Regulatory Networks [MESH] |*In Vitro Techniques [MESH] |Ebolavirus/classification/genetics [MESH] |Nucleic Acid Conformation [MESH] |Paper [MESH]Paper-based synthetic gene networks (epmc).pdf DeepDyve Pubget Overpricing