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2013 ; 3
(1
): 226-41
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Panning for Long Noncoding RNAs
#MMPMID24970166
Zhu S
; Zhang XO
; Yang L
Biomolecules
2013[Feb]; 3
(1
): 226-41
PMID24970166
show ga
The recent advent of high-throughput approaches has revealed widespread
transcription of the human genome, leading to a new appreciation of transcription
regulation, especially from noncoding regions. Distinct from most coding and
small noncoding RNAs, long noncoding RNAs (lncRNAs) are generally expressed at
low levels, are less conserved and lack protein-coding capacity. These intrinsic
features of lncRNAs have not only hampered their full annotation in the past
several years, but have also generated controversy concerning whether many or
most of these lncRNAs are simply the result of transcriptional noise. Here, we
assess these intrinsic features that have challenged lncRNA discovery and further
summarize recent progress in lncRNA discovery with integrated methodologies, from
which new lessons and insights can be derived to achieve better characterization
of lncRNA expression regulation. Full annotation of lncRNA repertoires and the
implications of such annotation will provide a fundamental basis for
comprehensive understanding of pervasive functions of lncRNAs in biological
regulation.