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2017 ; 12
(5
): e0178154
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PanWeb: A web interface for pan-genomic analysis
#MMPMID28542514
Pantoja Y
; Pinheiro K
; Veras A
; Araújo F
; Lopes de Sousa A
; Guimarães LC
; Silva A
; Ramos RTJ
PLoS One
2017[]; 12
(5
): e0178154
PMID28542514
show ga
With increased production of genomic data since the advent of next-generation
sequencing (NGS), there has been a need to develop new bioinformatics tools and
areas, such as comparative genomics. In comparative genomics, the genetic
material of an organism is directly compared to that of another organism to
better understand biological species. Moreover, the exponentially growing number
of deposited prokaryote genomes has enabled the investigation of several genomic
characteristics that are intrinsic to certain species. Thus, a new approach to
comparative genomics, termed pan-genomics, was developed. In pan-genomics,
various organisms of the same species or genus are compared. Currently, there are
many tools that can perform pan-genomic analyses, such as PGAP (Pan-Genome
Analysis Pipeline), Panseq (Pan-Genome Sequence Analysis Program) and PGAT
(Prokaryotic Genome Analysis Tool). Among these software tools, PGAP was
developed in the Perl scripting language and its reliance on UNIX platform
terminals and its requirement for an extensive parameterized command line can
become a problem for users without previous computational knowledge. Thus, the
aim of this study was to develop a web application, known as PanWeb, that serves
as a graphical interface for PGAP. In addition, using the output files of the
PGAP pipeline, the application generates graphics using custom-developed scripts
in the R programming language. PanWeb is freely available at
http://www.computationalbiology.ufpa.br/panweb.