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2007 ; 395
(ä): 177-86
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PSI-BLAST tutorial
#MMPMID17993673
Bhagwat M
; Aravind L
Methods Mol Biol
2007[]; 395
(ä): 177-86
PMID17993673
show ga
PSI-BLAST (Position-Specific Iterative Basic Local Alignment Search Tool) derives
a position-specific scoring matrix (PSSM) or profile from the multiple sequence
alignment of sequences detected above a given score threshold using
protein-protein BLAST. This PSSM is used to further search the database for new
matches, and is updated for subsequent iterations with these newly detected
sequences. Thus, PSI-BLAST provides a means of detecting distant relationships
between proteins. In this chapter, we discuss practical aspects of using
PSI-BLAST and provide a tutorial on how to uncover distant relationships between
proteins and use them to reach biologically meaningful conclusions.