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10.1016/j.gpb.2016.05.004 http://scihub22266oqcxt.onion/10.1016/j.gpb.2016.05.004 C5093776!5093776 !27646134     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27646134 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Genomics+Proteomics+Bioinformatics 2016 ; 14 (5 ): 265-279 Nephropedia Template TP {{tp|p=27646134 |t=2016. Oxford Nanopore MinION Sequencing and Genome Assembly |pdf=|usr=}}{{27646134 }} gab.com Text Oxford Nanopore MinION Sequencing and Genome Assembly JO: Genomics Proteomics Bioinformatics http://www.kidney.de/pget.php?&tw=1&pmid=27646134 #FOAvip The revolution of genome sequencing is continuing after the successful second-generation sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by Pacific Biosciences (PacBio), is progressing rapidly, moving from a technology once only capable of providing data for small genome analysis, or for performing targeted screening, to one that promises high quality de novo assembly and structural variation detection for human-sized genomes. In 2014, the MinION, the first commercial sequencer using nanopore technology, was released by Oxford Nanopore Technologies (ONT). MinION identifies DNA bases by measuring the changes in electrical conductivity generated as DNA strands pass through a biological pore. Its portability, affordability, and speed in data production makes it suitable for real-time applications, the release of the long read sequencer MinION has thus generated much excitement and interest in the genomics community. While de novo genome assemblies can be cheaply produced from SGS data, assembly continuity is often relatively poor, due to the limited ability of short reads to handle long repeats. Assembly quality can be greatly improved by using TGS long reads, since repetitive regions can be easily expanded into using longer sequencing lengths, despite having higher error rates at the base level. The potential of nanopore sequencing has been demonstrated by various studies in genome surveillance at locations where rapid and reliable sequencing is needed, but where resources are limited. Twit Text FOAVip Oxford Nanopore MinION Sequencing and Genome Assembly ????Genomics Proteomics Bioinformatics http://www.kidney.de/pget.php?&tw=1&pmid=27646134 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27646134 #FOAvip English Wikipedia <ref>{{Cite pmid|27646134 }}</ref> Oxford Nanopore MinION Sequencing and Genome Assembly #MMPMID27646134 Lu H ; Giordano F ; Ning Z Genomics Proteomics Bioinformatics 2016[Oct]; 14 (5 ): 265-279 PMID27646134 show ga The revolution of genome sequencing is continuing after the successful second-generation sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by Pacific Biosciences (PacBio), is progressing rapidly, moving from a technology once only capable of providing data for small genome analysis, or for performing targeted screening, to one that promises high quality de novo assembly and structural variation detection for human-sized genomes. In 2014, the MinION, the first commercial sequencer using nanopore technology, was released by Oxford Nanopore Technologies (ONT). MinION identifies DNA bases by measuring the changes in electrical conductivity generated as DNA strands pass through a biological pore. Its portability, affordability, and speed in data production makes it suitable for real-time applications, the release of the long read sequencer MinION has thus generated much excitement and interest in the genomics community. While de novo genome assemblies can be cheaply produced from SGS data, assembly continuity is often relatively poor, due to the limited ability of short reads to handle long repeats. Assembly quality can be greatly improved by using TGS long reads, since repetitive regions can be easily expanded into using longer sequencing lengths, despite having higher error rates at the base level. The potential of nanopore sequencing has been demonstrated by various studies in genome surveillance at locations where rapid and reliable sequencing is needed, but where resources are limited. |*Genome, Human [MESH] |High-Throughput Nucleotide Sequencing/instrumentation/*methods [MESH] |Humans [MESH] |Nanopores [MESH] |Repetitive Sequences, Nucleic Acid/*genetics [MESH]Oxford Nanopore MinION Sequencing and Genome Assembly (epmc).pdf DeepDyve Pubget Overpricing