Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.jss.2014.03.036 http://scihub22266oqcxt.onion/10.1016/j.jss.2014.03.036 C4225779!4225779 !24742622     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24742622 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24742622 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Surg+Res 2014 ; 190 (1 ): 55-63 Nephropedia Template TP {{tp|p=24742622 |t=2014. Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis |pdf=|usr=}}{{24742622 }} gab.com Text Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis JO: J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=24742622 #FOAvip BACKGROUND: Liver fibrosis is a common response to liver injury and, in severe cases, leads to cirrhosis. The hepatic stellate cells (HSCs) become activated after liver injury and play a significant role in fibrogenesis. The activated HSC is characterized by increased proliferation, overexpression of ? smooth muscle actin, and excessive production of extracellular matrix (ECM) proteins. Oridonin, a naturally occurring diterpenoid, has been shown to induce apoptosis in liver and gastric cancer cells. However, its effects on the HSC are unknown. METHODS: We tested the effects of oridonin on the activated human and rat HSC lines LX-2 and HSC-T6, and the human hepatocyte cell line C3A. Transforming growth factor ?1 (TGF-?1) was used to stimulate LX-2 cells. RESULTS: Oridonin significantly inhibited LX-2 and HSC-T6 proliferation. In contrast, oridonin had no antiproliferative effect on C3A cells at our tested range. Oridonin induced apoptosis and S-phase arrest in LX-2 cells. These findings were associated with an increase in p53, p21, p16, and cleaved Poly (ADP-ribose) Polymerase (PARP), and with a decrease in Cyclin-dependent kinase 4 (Cdk4). Oridonin markedly decreased expression of ? smooth muscle actin and ECM protein type I collagen and fibronectin, blocked TGF-?1-induced Smad2/3 phosphorylation and type I collagen expression. CONCLUSIONS: Oridonin induces apoptosis and cell cycle arrest involving the p53-p21 pathway in HSC and appears to be nontoxic to hepatocytes. In addition, oridonin suppressed endogenous and TGF-?1-induced ECM proteins. Thus, oridonin may act as a novel agent to prevent hepatic fibrosis. Twit Text FOAVip Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis ????J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=24742622 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24742622 #FOAvip English Wikipedia <ref>{{Cite pmid|24742622 }}</ref> Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis #MMPMID24742622 Bohanon FJ ; Wang X ; Ding C ; Ding Y ; Radhakrishnan GL ; Rastellini C ; Zhou J ; Radhakrishnan RS J Surg Res 2014[Jul]; 190 (1 ): 55-63 PMID24742622 show ga BACKGROUND: Liver fibrosis is a common response to liver injury and, in severe cases, leads to cirrhosis. The hepatic stellate cells (HSCs) become activated after liver injury and play a significant role in fibrogenesis. The activated HSC is characterized by increased proliferation, overexpression of ? smooth muscle actin, and excessive production of extracellular matrix (ECM) proteins. Oridonin, a naturally occurring diterpenoid, has been shown to induce apoptosis in liver and gastric cancer cells. However, its effects on the HSC are unknown. METHODS: We tested the effects of oridonin on the activated human and rat HSC lines LX-2 and HSC-T6, and the human hepatocyte cell line C3A. Transforming growth factor ?1 (TGF-?1) was used to stimulate LX-2 cells. RESULTS: Oridonin significantly inhibited LX-2 and HSC-T6 proliferation. In contrast, oridonin had no antiproliferative effect on C3A cells at our tested range. Oridonin induced apoptosis and S-phase arrest in LX-2 cells. These findings were associated with an increase in p53, p21, p16, and cleaved Poly (ADP-ribose) Polymerase (PARP), and with a decrease in Cyclin-dependent kinase 4 (Cdk4). Oridonin markedly decreased expression of ? smooth muscle actin and ECM protein type I collagen and fibronectin, blocked TGF-?1-induced Smad2/3 phosphorylation and type I collagen expression. CONCLUSIONS: Oridonin induces apoptosis and cell cycle arrest involving the p53-p21 pathway in HSC and appears to be nontoxic to hepatocytes. In addition, oridonin suppressed endogenous and TGF-?1-induced ECM proteins. Thus, oridonin may act as a novel agent to prevent hepatic fibrosis. |Actins/antagonists & inhibitors [MESH] |Animals [MESH] |Apoptosis/drug effects [MESH] |Cell Cycle Checkpoints/drug effects [MESH] |Cell Proliferation/*drug effects [MESH] |Cells, Cultured [MESH] |Diterpenes, Kaurane/*pharmacology [MESH] |Extracellular Matrix Proteins/analysis [MESH] |Hepatic Stellate Cells/*drug effects/physiology [MESH] |Humans [MESH] |Liver Cirrhosis/*prevention & control [MESH] |Rats [MESH]Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis(epmc).pdf DeepDyve Pubget Overpricing