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2014 ; 30
(ä): 112-20
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Optogenetic approaches to cell migration and beyond
#MMPMID25216352
Weitzman M
; Hahn KM
Curr Opin Cell Biol
2014[Oct]; 30
(ä): 112-20
PMID25216352
show ga
Optogenetics, the use of genetically encoded tools to control protein function
with light, can generate localized changes in signaling within living cells and
animals. For years it has been focused on channel proteins for neurobiology, but
has recently expanded to cover many different types of proteins, using a broad
array of different protein engineering approaches. These methods have largely
been directed at proteins involved in motility, cytoskeletal regulation and gene
expression. This review provides a survey of non-channel proteins that have been
engineered for optogenetics. Existing molecules are used to illustrate the
advantages and disadvantages of the many imaginative new approaches that the
reader can use to create light-controlled proteins.