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10.1038/nn.4161 http://scihub22266oqcxt.onion/10.1038/nn.4161 C4869830!4869830 !26551543     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26551543 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Neurosci 2015 ; 18 (12 ): 1845-1852 Nephropedia Template TP {{tp|p=26551543 |t=2015. Optogenetic acidification of synaptic vesicles and lysosomes |pdf=|usr=}}{{26551543 }} gab.com Text Optogenetic acidification of synaptic vesicles and lysosomes JO: Nat Neurosci http://www.kidney.de/pget.php?&tw=1&pmid=26551543 #FOAvip Acidification is required for the function of many intracellular organelles, but methods to acutely manipulate their intraluminal pH have not been available. Here we present a targeting strategy to selectively express the light-driven proton pump Arch3 on synaptic vesicles. Our new tool, pHoenix, can functionally replace endogenous proton pumps, enabling optogenetic control of vesicular acidification and neurotransmitter accumulation. Under physiological conditions, glutamatergic vesicles are nearly full, as additional vesicle acidification with pHoenix only slightly increased the quantal size. By contrast, we found that incompletely filled vesicles exhibited a lower release probability than full vesicles, suggesting preferential exocytosis of vesicles with high transmitter content. Our subcellular targeting approach can be transferred to other organelles, as demonstrated for a pHoenix variant that allows light-activated acidification of lysosomes. Twit Text FOAVip Optogenetic acidification of synaptic vesicles and lysosomes ????Nat Neurosci http://www.kidney.de/pget.php?&tw=1&pmid=26551543 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26551543 #FOAvip English Wikipedia <ref>{{Cite pmid|26551543 }}</ref> Optogenetic acidification of synaptic vesicles and lysosomes #MMPMID26551543 Rost BR ; Schneider F ; Grauel MK ; Wozny C ; Bentz C ; Blessing A ; Rosenmund T ; Jentsch TJ ; Schmitz D ; Hegemann P ; Rosenmund C Nat Neurosci 2015[Dec]; 18 (12 ): 1845-1852 PMID26551543 show ga Acidification is required for the function of many intracellular organelles, but methods to acutely manipulate their intraluminal pH have not been available. Here we present a targeting strategy to selectively express the light-driven proton pump Arch3 on synaptic vesicles. Our new tool, pHoenix, can functionally replace endogenous proton pumps, enabling optogenetic control of vesicular acidification and neurotransmitter accumulation. Under physiological conditions, glutamatergic vesicles are nearly full, as additional vesicle acidification with pHoenix only slightly increased the quantal size. By contrast, we found that incompletely filled vesicles exhibited a lower release probability than full vesicles, suggesting preferential exocytosis of vesicles with high transmitter content. Our subcellular targeting approach can be transferred to other organelles, as demonstrated for a pHoenix variant that allows light-activated acidification of lysosomes. |Animals [MESH] |Cells, Cultured [MESH] |Female [MESH] |HEK293 Cells [MESH] |Hippocampus/chemistry/cytology/metabolism [MESH] |Humans [MESH] |Lysosomes/*chemistry/*genetics/metabolism [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Optogenetics/*methods [MESH] |Rats [MESH]Optogenetic acidification of synaptic vesicles and lysosomes (epmc).pdf DeepDyve Pubget Overpricing