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10.1016/j.cels.2015.08.015 http://scihub22266oqcxt.onion/10.1016/j.cels.2015.08.015 C4669575!4669575 !26645048     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26645048 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Syst 2015 ; 1 (3 ): 210-223 Nephropedia Template TP {{tp|p=26645048 |t=2015. Optimizing cancer genome sequencing and analysis |pdf=|usr=}}{{26645048 }} gab.com Text Optimizing cancer genome sequencing and analysis JO: Cell Syst http://www.kidney.de/pget.php?&tw=1&pmid=26645048 #FOAvip Tumors are typically sequenced to depths of 75-100× (exome) or 30-50× (whole genome). We demonstrate that current sequencing paradigms are inadequate for tumors that are impure, aneuploid or clonally heterogeneous. To reassess optimal sequencing strategies, we performed ultra-deep (up to ~312×) whole genome sequencing (WGS) and exome capture (up to ~433×) of a primary acute myeloid leukemia, its subsequent relapse, and a matched normal skin sample. We tested multiple alignment and variant calling algorithms and validated ~200,000 putative SNVs by sequencing them to depths of ~1,000×. Additional targeted sequencing provided over 10,000× coverage and ddPCR assays provided up to ~250,000× sampling of selected sites. We evaluated the effects of different library generation approaches, depth of sequencing, and analysis strategies on the ability to effectively characterize a complex tumor. This dataset, representing the most comprehensively sequenced tumor described to date, will serve as an invaluable community resource (dbGaP accession id phs000159). Twit Text FOAVip Optimizing cancer genome sequencing and analysis ????Cell Syst http://www.kidney.de/pget.php?&tw=1&pmid=26645048 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26645048 #FOAvip English Wikipedia <ref>{{Cite pmid|26645048 }}</ref> Optimizing cancer genome sequencing and analysis #MMPMID26645048 Griffith M ; Miller CA ; Griffith OL ; Krysiak K ; Skidmore ZL ; Ramu A ; Walker JR ; Dang HX ; Trani L ; Larson DE ; Demeter RT ; Wendl MC ; McMichael JF ; Austin RE ; Magrini V ; McGrath SD ; Ly A ; Kulkarni S ; Cordes MG ; Fronick CC ; Fulton RS ; Maher CA ; Ding L ; Klco JM ; Mardis ER ; Ley TJ ; Wilson RK Cell Syst 2015[Sep]; 1 (3 ): 210-223 PMID26645048 show ga Tumors are typically sequenced to depths of 75-100× (exome) or 30-50× (whole genome). We demonstrate that current sequencing paradigms are inadequate for tumors that are impure, aneuploid or clonally heterogeneous. To reassess optimal sequencing strategies, we performed ultra-deep (up to ~312×) whole genome sequencing (WGS) and exome capture (up to ~433×) of a primary acute myeloid leukemia, its subsequent relapse, and a matched normal skin sample. We tested multiple alignment and variant calling algorithms and validated ~200,000 putative SNVs by sequencing them to depths of ~1,000×. Additional targeted sequencing provided over 10,000× coverage and ddPCR assays provided up to ~250,000× sampling of selected sites. We evaluated the effects of different library generation approaches, depth of sequencing, and analysis strategies on the ability to effectively characterize a complex tumor. This dataset, representing the most comprehensively sequenced tumor described to date, will serve as an invaluable community resource (dbGaP accession id phs000159). äOptimizing cancer genome sequencing and analysis (epmc).pdf DeepDyve Pubget Overpricing