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2017 ; 22
(1
): 31
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Optimized protocol for whole organ decellularization
#MMPMID28886732
Schmitt A
; Csiki R
; Tron A
; Saldamli B
; Tübel J
; Florian K
; Siebenlist S
; Balmayor E
; Burgkart R
Eur J Med Res
2017[Sep]; 22
(1
): 31
PMID28886732
show ga
BACKGROUND: The idea of tissue decellularization to gain matrices for tissue
engineering is promising. The aim of the present study is to establish a safe and
reproducible protocol for solid tissue decellularization that prevents the
architecture of the matrix with the inherent vascular network. METHODS: The study
was performed in rat kidneys which were decellularized by a SDS-based perfusion
protocol. Perfusion time and SDS concentration were systematically changed to
obtain the shortest and most gentle protocol that leads to complete
decellularization. RESULTS: We investigated kinetics of protein elution,
decellularization success, and remaining cell toxicity. This resulted in a
reproducible protocol, leading to safe decellularization with prevention of the
inherent vascular network, without remaining detectable cell toxicity. The
established protocol leads to solid tissue decellularization in only 7 h, which
is by far shorter than the previously published methods. CONCLUSION: The
established technique has the potential to become a relevant platform technology
for tissue engineering of solid tissues. It provides a solution for the
yet-unsolved problem of vascularization.
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