Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25592419
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Science
2015 ; 347
(6221
): 543-8
Nephropedia Template TP
Science
2015[Jan]; 347
(6221
): 543-8
PMID25592419
show ga
In optical microscopy, fine structural details are resolved by using refraction
to magnify images of a specimen. We discovered that by synthesizing a swellable
polymer network within a specimen, it can be physically expanded, resulting in
physical magnification. By covalently anchoring specific labels located within
the specimen directly to the polymer network, labels spaced closer than the
optical diffraction limit can be isotropically separated and optically resolved,
a process we call expansion microscopy (ExM). Thus, this process can be used to
perform scalable superresolution microscopy with diffraction-limited microscopes.
We demonstrate ExM with apparent ~70-nanometer lateral resolution in both
cultured cells and brain tissue, performing three-color superresolution imaging
of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal
microscope.
free
free
free
