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2016 ; 21
(5
): 50902
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English Wikipedia
Optical coherence tomography for embryonic imaging: a review
#MMPMID27228503
Raghunathan R
; Singh M
; Dickinson ME
; Larin KV
J Biomed Opt
2016[May]; 21
(5
): 50902
PMID27228503
show ga
Embryogenesis is a highly complex and dynamic process, and its visualization is
crucial for understanding basic physiological processes during development and
for identifying and assessing possible defects, malformations, and diseases.
While traditional imaging modalities, such as ultrasound biomicroscopy,
micro-magnetic resonance imaging, and micro-computed tomography, have long been
adapted for embryonic imaging, these techniques generally have limitations in
their speed, spatial resolution, and contrast to capture processes such as
cardiodynamics during embryogenesis. Optical coherence tomography (OCT) is a
noninvasive imaging modality with micrometer-scale spatial resolution and imaging
depth up to a few millimeters in tissue. OCT has bridged the gap between
ultrahigh resolution imaging techniques with limited imaging depth like confocal
microscopy and modalities, such as ultrasound sonography, which have deeper
penetration but poorer spatial resolution. Moreover, the noninvasive nature of
OCT has enabled live imaging of embryos without any external contrast agents. We
review how OCT has been utilized to study developing embryos and also discuss
advances in techniques used in conjunction with OCT to understand embryonic
development.