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2017 ; 7
(1
): 9195
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Optical Control of Tumor Induction in the Zebrafish
#MMPMID28835665
Feng Z
; Nam S
; Hamouri F
; Aujard I
; Ducos B
; Vriz S
; Volovitch M
; Jullien L
; Lin S
; Weiss S
; Bensimon D
Sci Rep
2017[Aug]; 7
(1
): 9195
PMID28835665
show ga
The zebrafish has become an increasingly popular and valuable cancer model over
the past few decades. While most zebrafish cancer models are generated by
expressing mammalian oncogenes under tissue-specific promoters, here we describe
a method that allows for the precise optical control of oncogene expression in
live zebrafish. We utilize this technique to transiently or constitutively
activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate
the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal
control of oncogene expression in live zebrafish, and characterize the different
tumorigenic probabilities when kRASG12V is expressed transiently or
constitutively at different developmental stages. Moreover, we show that light
can be used to activate oncogene expression in selected tissues and single cells
without tissue-specific promoters. Our work presents a novel approach to initiate
and study cancer in zebrafish, and the high spatiotemporal resolution of this
method makes it a valuable tool for studying cancer initiation from single cells.
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