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2015 ; 88
(1
): 15-22
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On the Collagen Mineralization A Review
#MMPMID26528042
Tomoaia G
; Pasca RD
Clujul Med
2015[]; 88
(1
): 15-22
PMID26528042
show ga
Collagen mineralization (CM) is a challenging process that has received a lot of
attention in the past years. Among the reasons for this interest, the key role is
the importance of collagen and hydroxyapatite in natural bone, as major
constituents. Different protocols of mineralization have been developed,
specially using simulated body fluid (SBF) and many methods have been used to
characterize the systems obtained, starting with methods of determining the
mineral content (XRD, FTIR, Raman, High-Resolution Spectral Ultrasound Imaging),
continuing with imaging methods (AFM, TEM, SEM, Fluorescence Microscopy), thermal
analysis (DSC and TGA), evaluation of the mechanical and biological properties,
including statistical methods and molecular modeling. In spite of the great
number of studies regarding collagen mineralization, its mechanism, both in vivo
and in vitro, is not completely understood. Some of the methods used in vitro and
investigation methods are reviewed here.
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