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10.1038/mtna.2015.37 http://scihub22266oqcxt.onion/10.1038/mtna.2015.37 C4877446!4877446 !26575098     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26575098 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Ther+Nucleic+Acids 2015 ; 4 (11 ): e264 Nephropedia Template TP {{tp|p=26575098 |t=2015. Off-target Effects in CRISPR/Cas9-mediated Genome Engineering |pdf=|usr=}}{{26575098 }} gab.com Text Off-target Effects in CRISPR/Cas9-mediated Genome Engineering JO: Mol Ther Nucleic Acids http://www.kidney.de/pget.php?&tw=1&pmid=26575098 #FOAvip CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functionality of genetic elements, creating genetically modified organisms as well as preclinical research of genetic disorders. However, the high frequency of off-target activity (?50%)-RGEN (RNA-guided endonuclease)-induced mutations at sites other than the intended on-target site-is one major concern, especially for therapeutic and clinical applications. Here, we review the basic mechanisms underlying off-target cutting in the CRISPR/Cas9 system, methods for detecting off-target mutations, and strategies for minimizing off-target cleavage. The improvement off-target specificity in the CRISPR/Cas9 system will provide solid genotype-phenotype correlations, and thus enable faithful interpretation of genome-editing data, which will certainly facilitate the basic and clinical application of this technology. Twit Text FOAVip Off-target Effects in CRISPR/Cas9-mediated Genome Engineering ????Mol Ther Nucleic Acids http://www.kidney.de/pget.php?&tw=1&pmid=26575098 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26575098 #FOAvip English Wikipedia <ref>{{Cite pmid|26575098 }}</ref> Off-target Effects in CRISPR/Cas9-mediated Genome Engineering #MMPMID26575098 Zhang XH ; Tee LY ; Wang XG ; Huang QS ; Yang SH Mol Ther Nucleic Acids 2015[Nov]; 4 (11 ): e264 PMID26575098 show ga CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functionality of genetic elements, creating genetically modified organisms as well as preclinical research of genetic disorders. However, the high frequency of off-target activity (?50%)-RGEN (RNA-guided endonuclease)-induced mutations at sites other than the intended on-target site-is one major concern, especially for therapeutic and clinical applications. Here, we review the basic mechanisms underlying off-target cutting in the CRISPR/Cas9 system, methods for detecting off-target mutations, and strategies for minimizing off-target cleavage. The improvement off-target specificity in the CRISPR/Cas9 system will provide solid genotype-phenotype correlations, and thus enable faithful interpretation of genome-editing data, which will certainly facilitate the basic and clinical application of this technology. äOff-target Effects in CRISPR/Cas9-mediated Genome Engineering (epmc).pdf DeepDyve Pubget Overpricing