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2014 ; 13
(1
): 21-28
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Observation of spermatozoa by a high-magnification microscope
#MMPMID29662368
Komiya A
; Watanabe A
; Kato T
; Kawauchi Y
; Fuse H
Reprod Med Biol
2014[Jan]; 13
(1
): 21-28
PMID29662368
show ga
Semen analyses are the primary tool for evaluating male infertility, as semen
parameters are useful for predicting potential fertility. In the field of
assisted reproductive technology (ART), the single best motile spermatozoon
should be selected, especially when performing intracytoplasmic sperm injection
(ICSI). In this context, the motile sperm organelle morphology examination
(MSOME) was developed as a method of assessing the detailed morphology of motile
spermatozoa in real time at a magnification of up to 6,300× on a video system.
The use of ICSI with MSOME-selected sperm is called intracytoplasmic
morphologically selected sperm injection (IMSI). IMSI improves the outcomes of
ICSI. MSOME can be also applied to evaluate male infertility. Among MSOME
parameters, the presence of sperm nuclear vacuoles is the most important finding.
Large sperm nuclear vacuoles (LNV) are related not only to poor ART outcomes, but
also to poor semen quality and sperm DNA damage, such as DNA fragmentation and
chromatin condensation failure. It has been suggested that sperm head vacuoles
are produced at earlier stages of sperm maturation. It is possible that the
number of LNV can be decreased by surgical or medical treatment for male
infertility. Therefore, the level of LNV has the potential to be used as an
alternative parameter of semen quality and a new tool for evaluating the
therapeutic effects of treatment in male patients with infertility.