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2016 ; 30
(6
): 660-72
Nephropedia Template TP
Genes Dev
2016[Mar]; 30
(6
): 660-72
PMID26966245
show ga
Nucleosome repositioning at gene promoters is a fundamental aspect of the
regulation of gene expression. However, the extent to which nucleosome
repositioning is used within eukaryotic genomes is poorly understood. Here we
report a comprehensive analysis of nucleosome positions as budding yeast transit
through an ultradian cycle in which expression of >50% of all genes is highly
synchronized. We present evidence of extensive nucleosome repositioning at
thousands of gene promoters as genes are activated and repressed. During
activation, nucleosomes are relocated to allow sites of general transcription
factor binding and transcription initiation to become accessible. The extent of
nucleosome shifting is closely related to the dynamic range of gene transcription
and generally related to DNA sequence properties and use of the coactivators
TFIID or SAGA. However, dynamic gene expression is not limited to SAGA-regulated
promoters and is an inherent feature of most genes. While nucleosome
repositioning occurs pervasively, we found that a class of genes required for
growth experience acute nucleosome shifting as cells enter the cell cycle.
Significantly, our data identify that the ATP-dependent chromatin-remodeling
enzyme Snf2 plays a fundamental role in nucleosome repositioning and the
expression of growth genes. We also reveal that nucleosome organization changes
extensively in concert with phases of the cell cycle, with large, regularly
spaced nucleosome arrays being established in mitosis. Collectively, our data and
analysis provide a framework for understanding nucleosome dynamics in relation to
fundamental DNA-dependent transactions.
|*Gene Expression Regulation, Fungal
[MESH]
|Base Sequence/genetics
[MESH]
|Chromatin Assembly and Disassembly/genetics
[MESH]
free
free
free
