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10.7554/eLife.13450 http://scihub22266oqcxt.onion/10.7554/eLife.13450 C4880442!4880442 !27130520     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27130520 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Elife 2016 ; 5 (ä): ä Nephropedia Template TP {{tp|p=27130520 |t=2016. Nucleosome breathing and remodeling constrain CRISPR-Cas9 function |pdf=|usr=}}{{27130520 }} gab.com Text Nucleosome breathing and remodeling constrain CRISPR-Cas9 function JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=27130520 #FOAvip The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains the activity of the CRISPR-Cas9 system. We find that nucleosomes assembled on native DNA sequences are permissive to Cas9 action. However, the accessibility of nucleosomal DNA to Cas9 is variable over several orders of magnitude depending on dynamic properties of the DNA sequence and the distance of the PAM site from the nucleosome dyad. We further find that chromatin remodeling enzymes stimulate Cas9 activity on nucleosomal templates. Our findings imply that the spontaneous breathing of nucleosomal DNA together with the action of chromatin remodelers allow Cas9 to effectively act on chromatin in vivo. Twit Text FOAVip Nucleosome breathing and remodeling constrain CRISPR-Cas9 function ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=27130520 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27130520 #FOAvip English Wikipedia <ref>{{Cite pmid|27130520 }}</ref> Nucleosome breathing and remodeling constrain CRISPR-Cas9 function #MMPMID27130520 Isaac RS ; Jiang F ; Doudna JA ; Lim WA ; Narlikar GJ ; Almeida R Elife 2016[Apr]; 5 (ä): ä PMID27130520 show ga The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains the activity of the CRISPR-Cas9 system. We find that nucleosomes assembled on native DNA sequences are permissive to Cas9 action. However, the accessibility of nucleosomal DNA to Cas9 is variable over several orders of magnitude depending on dynamic properties of the DNA sequence and the distance of the PAM site from the nucleosome dyad. We further find that chromatin remodeling enzymes stimulate Cas9 activity on nucleosomal templates. Our findings imply that the spontaneous breathing of nucleosomal DNA together with the action of chromatin remodelers allow Cas9 to effectively act on chromatin in vivo. |*CRISPR-Cas Systems [MESH] |*Recombination, Genetic [MESH] |Animals [MESH] |Bacterial Proteins/*metabolism [MESH] |CRISPR-Associated Protein 9 [MESH] |DNA/*metabolism [MESH] |Endonucleases/*metabolism [MESH] |Gene Editing/*methods [MESH] |Nucleosomes/*metabolism [MESH] |Protein Binding [MESH]Nucleosome breathing and remodeling constrain CRISPR-Cas9 function (epmc).pdf DeepDyve Pubget Overpricing