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2012 ; 2012
(ä): 245706
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Nucleosome Positioning
#MMPMID27335664
Nishida H
ISRN Mol Biol
2012[]; 2012
(ä): 245706
PMID27335664
show ga
Nucleosome positioning is not only related to genomic DNA compaction but also to
other biological functions. After the chromatin is digested by micrococcal
nuclease, nucleosomal (nucleosome-bound) DNA fragments can be sequenced and
mapped on the genomic DNA sequence. Due to the development of modern DNA
sequencing technology, genome-wide nucleosome mapping has been performed in a
wide range of eukaryotic species. Comparative analyses of the nucleosome
positions have revealed that the nucleosome is more frequently formed in exonic
than intronic regions, and that most of transcription start and translation (or
transcription) end sites are located in nucleosome linker DNA regions, indicating
that nucleosome positioning influences transcription initiation, transcription
termination, and gene splicing. In addition, nucleosomal DNA contains guanine and
cytosine (G + C)-rich sequences and a high level of cytosine methylation. Thus,
the nucleosome positioning system has been conserved during eukaryotic evolution.