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2014 ; 111
(50
): 17893-8
Nephropedia Template TP
Proc Natl Acad Sci U S A
2014[Dec]; 111
(50
): 17893-8
PMID25468975
show ga
Gene product molecule numbers fluctuate over time and between cells, confounding
deterministic expectations. The molecular origins of this noise of gene
expression remain unknown. Recent EM analysis of single PHO5 gene molecules of
yeast indicated that promoter molecules stochastically assume alternative
nucleosome configurations at steady state, including the fully nucleosomal and
nucleosome-free configuration. Given that distinct configurations are unequally
conducive to transcription, the nucleosomal variation of promoter molecules may
constitute a source of gene expression noise. This notion, however, implies an
untested conjecture, namely that the nucleosomal variation arises de novo or
intrinsically (i.e., that it cannot be explained as the result of the promoter's
deterministic response to variation in its molecular surroundings). Here, we
show--by microscopically analyzing the nucleosome configurations of two
juxtaposed physically linked PHO5 promoter copies--that the configurational
variation, indeed, is intrinsically stochastic and thus, a cause of gene
expression noise rather than its effect.
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