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10.1016/j.chroma.2016.02.057 http://scihub22266oqcxt.onion/10.1016/j.chroma.2016.02.057 C4801196!4801196 !26948761     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26948761 &cmd=llinks): Failed to open stream: HTTP request failed! 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Nucleic acid separations using superficially porous silica particles |pdf=|usr=}}{{26948761 }} gab.com Text Nucleic acid separations using superficially porous silica particles JO: J Chromatogr A http://www.kidney.de/pget.php?&tw=1&pmid=26948761 #FOAvip Ion pair reverse-phase liquid chromatography has been widely employed for nucleic acid separations. A wide range of alternative stationary phases have been utilised in conjunction with ion pair reverse-phase chromatography, including totally porous particles, non-porous particles, macroporous particles and monolithic stationary phases. In this study we have utilised superficially porous silica particles in conjunction with ion pair reverse-phase liquid chromatography for the analysis of nucleic acids. We have investigated a range of different pore-sizes and phases for the analysis of a diverse range of nucleic acids including oligonucleotides, oligoribonucleotides, phosphorothioate oligonucleotides and high molecular weight dsDNA and RNA. The pore size of the superficially porous silica particles was shown to significantly affect the resolution of the nucleic acids. Optimum separations of small oligonucleotides such as those generated in RNase mapping experiments were obtained with 80Å pore sizes and can readily be interfaced with mass spectrometry analysis. Improved resolution of larger oligonucleotides (>19mers) was observed with pore sizes of 150Å. The optimum resolution for larger dsDNA/RNA molecules was achieved using superficially porous silica particles with pore sizes of 400Å. Furthermore, we have utilised 150Å pore size solid-core particles to separate typical impurities of a fully phosphorothioated oligonucleotide, which are often generated in the synthesis of this important class of therapeutic oligonucleotide. Twit Text FOAVip Nucleic acid separations using superficially porous silica particles ????J Chromatogr A http://www.kidney.de/pget.php?&tw=1&pmid=26948761 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26948761 #FOAvip English Wikipedia <ref>{{Cite pmid|26948761 }}</ref> Nucleic acid separations using superficially porous silica particles #MMPMID26948761 Close ED ; Nwokeoji AO ; Milton D ; Cook K ; Hindocha DM ; Hook EC ; Wood H ; Dickman MJ J Chromatogr A 2016[Apr]; 1440 (ä): 135-144 PMID26948761 show ga Ion pair reverse-phase liquid chromatography has been widely employed for nucleic acid separations. A wide range of alternative stationary phases have been utilised in conjunction with ion pair reverse-phase chromatography, including totally porous particles, non-porous particles, macroporous particles and monolithic stationary phases. In this study we have utilised superficially porous silica particles in conjunction with ion pair reverse-phase liquid chromatography for the analysis of nucleic acids. We have investigated a range of different pore-sizes and phases for the analysis of a diverse range of nucleic acids including oligonucleotides, oligoribonucleotides, phosphorothioate oligonucleotides and high molecular weight dsDNA and RNA. The pore size of the superficially porous silica particles was shown to significantly affect the resolution of the nucleic acids. Optimum separations of small oligonucleotides such as those generated in RNase mapping experiments were obtained with 80Å pore sizes and can readily be interfaced with mass spectrometry analysis. Improved resolution of larger oligonucleotides (>19mers) was observed with pore sizes of 150Å. The optimum resolution for larger dsDNA/RNA molecules was achieved using superficially porous silica particles with pore sizes of 400Å. Furthermore, we have utilised 150Å pore size solid-core particles to separate typical impurities of a fully phosphorothioated oligonucleotide, which are often generated in the synthesis of this important class of therapeutic oligonucleotide. |Chemistry Techniques, Analytical/*methods/standards [MESH] |Chromatography, Reverse-Phase [MESH] |Mass Spectrometry [MESH] |Nucleic Acids/*isolation & purification [MESH] |Particle Size [MESH] |Porosity [MESH]Nucleic acid separations using superficially porous silica particles (epmc).pdf DeepDyve Pubget Overpricing