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10.1016/j.btre.2016.10.001 http://scihub22266oqcxt.onion/10.1016/j.btre.2016.10.001 C5361071!5361071 !28352552     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28352552 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biotechnol+Rep+(Amst) 2016 ; 12 (ä): 33-39 Nephropedia Template TP {{tp|p=28352552 |t=2016. Nucleic acid protocols: Extraction and optimization |pdf=|usr=}}{{28352552 }} gab.com Text Nucleic acid protocols: Extraction and optimization JO: Biotechnol Rep (Amst) http://www.kidney.de/pget.php?&tw=1&pmid=28352552 #FOAvip Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, and toxic material free protocol for extracting DNA and RNA from different prokaryotic and eukaryotic sources exploiting the physical and chemical properties of nucleic acids. Furthermore, this protocol showed that DNA and RNA are under triple protection (i.e. EDTA, SDS and NaCl) during lysis step, and this environment is improper for RNase to have DNA liberated of RNA and even for DNase to degrade the DNA. Therefore, the complete removal of RNA under RNase influence is achieved when RNase is added after DNA extraction, which gives optimal quality with any protocols. Similarly, DNA contamination in an isolated RNA is degraded by DNase to obtain high-quality RNA. Our protocol is the protocol of choice in terms of simplicity, recovery time, environmental safety, amount, purity, PCR and RT-PCR applicability. Twit Text FOAVip Nucleic acid protocols: Extraction and optimization ????Biotechnol Rep (Amst) http://www.kidney.de/pget.php?&tw=1&pmid=28352552 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28352552 #FOAvip English Wikipedia <ref>{{Cite pmid|28352552 }}</ref> Nucleic acid protocols: Extraction and optimization #MMPMID28352552 El-Ashram S ; Al Nasr I ; Suo X Biotechnol Rep (Amst) 2016[Dec]; 12 (ä): 33-39 PMID28352552 show ga Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, and toxic material free protocol for extracting DNA and RNA from different prokaryotic and eukaryotic sources exploiting the physical and chemical properties of nucleic acids. Furthermore, this protocol showed that DNA and RNA are under triple protection (i.e. EDTA, SDS and NaCl) during lysis step, and this environment is improper for RNase to have DNA liberated of RNA and even for DNase to degrade the DNA. Therefore, the complete removal of RNA under RNase influence is achieved when RNase is added after DNA extraction, which gives optimal quality with any protocols. Similarly, DNA contamination in an isolated RNA is degraded by DNase to obtain high-quality RNA. Our protocol is the protocol of choice in terms of simplicity, recovery time, environmental safety, amount, purity, PCR and RT-PCR applicability. äNucleic acid protocols: Extraction and optimization (epmc).pdf DeepDyve Pubget Overpricing