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2012 ; 4
(4
): 323-334
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Nonlinear optical microscopy in decoding arterial diseases
#MMPMID28510209
Ko AC
; Ridsdale A
; Mostaço-Guidolin LB
; Major A
; Stolow A
; Sowa MG
Biophys Rev
2012[Dec]; 4
(4
): 323-334
PMID28510209
show ga
Pathological understanding of arterial diseases is mainly attributable to
histological observations based on conventional tissue staining protocols. The
emerging development of nonlinear optical microscopy (NLOM), particularly in
second-harmonic generation, two-photon excited fluorescence and coherent Raman
scattering, provides a new venue to visualize pathological changes in the
extracellular matrix caused by atherosclerosis progression. These techniques in
general require minimal tissue preparation and offer rapid three-dimensional
imaging. The capability of label-free microscopic imaging enables disease impact
to be studied directly on the bulk artery tissue, thus minimally perturbing the
sample. In this review, we look at recent progress in applications related to
arterial disease imaging using various forms of NLOM.