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10.1073/pnas.1615375114 http://scihub22266oqcxt.onion/10.1073/pnas.1615375114 C5347600!5347600 !28223521     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28223521 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2017 ; 114 (10 ): E1866-E1874 Nephropedia Template TP {{tp|p=28223521 |t=2017. Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring |pdf=|usr=}}{{28223521 }} gab.com Text Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=28223521 #FOAvip Here, we report a method for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and mRNA from a variety of cell types. Cytosolic contents were repeatedly sampled from the same cell or population of cells for more than 5 d through a cell-culture substrate, incorporating hollow 150-nm-diameter nanostraws (NS) within a defined sampling region. Once extracted, the cellular contents were analyzed with conventional methods, including fluorescence, enzymatic assays (ELISA), and quantitative real-time PCR. This process was nondestructive with >95% cell viability after sampling, enabling long-term analysis. It is important to note that the measured quantities from the cell extract were found to constitute a statistically significant representation of the actual contents within the cells. Of 48 mRNA sequences analyzed from a population of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs), 41 were accurately quantified. The NS platform samples from a select subpopulation of cells within a larger culture, allowing native cell-to-cell contact and communication even during vigorous activity such as cardiomyocyte beating. This platform was applied both to cell lines and to primary cells, including CHO cells, hiPSC-CMs, and human astrocytes derived in 3D cortical spheroids. By tracking the same cell or group of cells over time, this method offers an avenue to understand dynamic cell behavior, including processes such as induced pluripotency and differentiation. Twit Text FOAVip Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=28223521 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28223521 #FOAvip English Wikipedia <ref>{{Cite pmid|28223521 }}</ref> Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring #MMPMID28223521 Cao Y ; Hjort M ; Chen H ; Birey F ; Leal-Ortiz SA ; Han CM ; Santiago JG ; Pa?ca SP ; Wu JC ; Melosh NA Proc Natl Acad Sci U S A 2017[Mar]; 114 (10 ): E1866-E1874 PMID28223521 show ga Here, we report a method for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and mRNA from a variety of cell types. Cytosolic contents were repeatedly sampled from the same cell or population of cells for more than 5 d through a cell-culture substrate, incorporating hollow 150-nm-diameter nanostraws (NS) within a defined sampling region. Once extracted, the cellular contents were analyzed with conventional methods, including fluorescence, enzymatic assays (ELISA), and quantitative real-time PCR. This process was nondestructive with >95% cell viability after sampling, enabling long-term analysis. It is important to note that the measured quantities from the cell extract were found to constitute a statistically significant representation of the actual contents within the cells. Of 48 mRNA sequences analyzed from a population of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs), 41 were accurately quantified. The NS platform samples from a select subpopulation of cells within a larger culture, allowing native cell-to-cell contact and communication even during vigorous activity such as cardiomyocyte beating. This platform was applied both to cell lines and to primary cells, including CHO cells, hiPSC-CMs, and human astrocytes derived in 3D cortical spheroids. By tracking the same cell or group of cells over time, this method offers an avenue to understand dynamic cell behavior, including processes such as induced pluripotency and differentiation. |Animals [MESH] |CHO Cells [MESH] |Cell Differentiation/drug effects [MESH] |Cell Tracking/*methods [MESH] |Cricetulus [MESH] |Cytoplasm/chemistry/drug effects [MESH] |Embryonic Stem Cells/*chemistry/cytology [MESH] |Humans [MESH] |Induced Pluripotent Stem Cells/chemistry/cytology [MESH] |Myocytes, Cardiac/chemistry/cytology [MESH] |Proteins/chemistry/*isolation & purification [MESH]Nondestructive nanostraw intracellular sampling for longitudinal cell (epmc).pdf DeepDyve Pubget Overpricing