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Natural polyreactive IgA antibodies coat the intestinal microbiota
#MMPMID28971969
Bunker JJ
; Erickson SA
; Flynn TM
; Henry C
; Koval JC
; Meisel M
; Jabri B
; Antonopoulos DA
; Wilson PC
; Bendelac A
Science
2017[Oct]; 358
(6361
): ? PMID28971969
show ga
Large quantities of immunoglobulin A (IgA) are constitutively secreted by
intestinal plasma cells to coat and contain the commensal microbiota, yet the
specificity of these antibodies remains elusive. Here we profiled the
reactivities of single murine IgA plasma cells by cloning and characterizing
large numbers of monoclonal antibodies. IgAs were not specific to individual
bacterial taxa but rather polyreactive, with broad reactivity to a diverse, but
defined, subset of microbiota. These antibodies arose at low frequencies among
naïve B cells and were selected into the IgA repertoire upon recirculation in
Peyer's patches. This selection process occurred independent of microbiota or
dietary antigens. Furthermore, although some IgAs acquired somatic mutations,
these did not substantially influence their reactivity. These findings reveal an
endogenous mechanism driving homeostatic production of polyreactive IgAs with
innate specificity to microbiota.
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