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2016 ; 8
(9
): 929-35
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Nanoscale mechanics guides cellular decision making
#MMPMID27477049
Rahil Z
; Pedron S
; Wang X
; Ha T
; Harley B
; Leckband D
Integr Biol (Camb)
2016[Sep]; 8
(9
): 929-35
PMID27477049
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This study used novel, force-limited nanoscale tension gauges to investigate how
force and substrate stiffness guide cellular decision-making during initial cell
attachment and spreading on deformable substrates. The well-established
dependence of cell traction and spreading on substrate stiffness has been
attributed to levels of force exerted on molecular components in focal contacts.
The molecular tension gauges used in this study enabled direct estimates of
threshold, pico Newton forces that instructed decision-making at different stages
of cell attachment, spreading, and adhesion maturation. Results show that the
force thresholds controlling adhesion and spreading transitions depend on
substrate stiffness. Reported findings agree qualitatively with a proposed model
that attributes rigidity-dependent differences in cell spreading to
stiffness-dependent rates of competing biochemical processes. Moreover, estimated
magnitudes of force thresholds governing transitions in cell attachment and
spreading, based on these in situ measurements, were in remarkable agreement with
prior less direct measurements.
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