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2015 ; 209
(5
): 633-44
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Nanobodies and recombinant binders in cell biology
#MMPMID26056137
Helma J
; Cardoso MC
; Muyldermans S
; Leonhardt H
J Cell Biol
2015[Jun]; 209
(5
): 633-44
PMID26056137
show ga
Antibodies are key reagents to investigate cellular processes. The development of
recombinant antibodies and binders derived from natural protein scaffolds has
expanded traditional applications, such as immunofluorescence, binding arrays,
and immunoprecipitation. In addition, their small size and high stability in
ectopic environments have enabled their use in all areas of cell research,
including structural biology, advanced microscopy, and intracellular expression.
Understanding these novel reagents as genetic modules that can be integrated into
cellular pathways opens up a broad experimental spectrum to monitor and
manipulate cellular processes.