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2014 ; 10
(6
): 1175-83
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Mycobacteria inactivation using Engineered Water Nanostructures (EWNS)
#MMPMID24632246
Pyrgiotakis G
; McDevitt J
; Gao Y
; Branco A
; Eleftheriadou M
; Lemos B
; Nardell E
; Demokritou P
Nanomedicine
2014[Aug]; 10
(6
): 1175-83
PMID24632246
show ga
Airborne transmitted pathogens such as Mycobacterium tuberculosis (Mtb) cause
serious, often fatal infectious disease with enormous global health implications.
Due to their unique cell wall and slow growth, mycobacteria are among the most
resilient microbial forms. Herein we evaluate the ability of an emerging,
chemical-free, nanotechnology-based method to inactivate M. parafortuitum (Mtb
surrogate). This method is based on the transformation of atmospheric water vapor
into engineered water nano-structures (EWNS) via electrospray. We demonstrate
that the EWNS can interact with and inactivate airborne mycobacteria, reducing
their concentration levels significantly. Additionally, EWNS can inactivate M.
parafortuitum on surfaces eight times faster than the control. The mechanism of
mycobacteria inactivation was also investigated in this study. It was
demonstrated that the EWNS effectively deliver the reactive oxygen species,
encapsulated during the electrospray process, to the bacteria oxidizing their
cell membrane resulting into inactivation. Overall, this is a method with the
potential to become an effective intervention technology in the battle against
airborne infections. FROM THE CLINICAL EDITOR: This study demonstrates the
feasibility of mycobacterium inactivation in airborne form or on contact surfaces
using electrospray activated water nano-structures. Given that the method is free
of toxic chemicals, this might become an important tool in the prevention of
mycobacterial infections, which are notoriously hard to treat.