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2012 ; 35
(1
): 51-5
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Multispectral enhancement towards digital staining
#MMPMID22002722
Bautista PA
; Yagi Y
Anal Cell Pathol (Amst)
2012[]; 35
(1
): 51-5
PMID22002722
show ga
BACKGROUND: Digital staining can be considered as a special form of image
enhancement wherein the concern is not only to increase the contrast between the
background objects and objects of interest, but to also impart the colors that
mark the objects' unique reactions to a specific stain. In this paper, we
extended the previously proposed multispectral enhancement methods such that the
colors of the background pixels can also be changed. METHODS: In the previous
multispectral enhancement methods a shifting factor is provided to the original
spectrum. To implement digital staining, a spectral transformation process is
introduced prior to spectral shifting. RESULTS: The enhancement method is applied
to multispectral images of H&E stained liver tissue. The resulting digitally
stained images show good correlation with the serial-section images of the tissue
which are physically stained with Masson's trichrome. CONCLUSIONS: We have
presented a multispectral enhancement method that can be adjusted to produce
digitally stained-images. The current experimental results show the viability of
the method. However, to achieve robust enhancement performance issues that arise
from variations in staining conditions has to be addressed as well. This would be
part of our future work.