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10.1371/journal.pone.0121674 http://scihub22266oqcxt.onion/10.1371/journal.pone.0121674 C4372376!4372376 !25803711     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25803711 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2015 ; 10 (3 ): e0121674 Nephropedia Template TP {{tp|p=25803711 |t=2015. Multimodal holographic microscopy: distinction between apoptosis and oncosis |pdf=|usr=}}{{25803711 }} gab.com Text Multimodal holographic microscopy: distinction between apoptosis and oncosis JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25803711 #FOAvip Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI- phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis. Twit Text FOAVip Multimodal holographic microscopy: distinction between apoptosis and oncosis ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25803711 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25803711 #FOAvip English Wikipedia <ref>{{Cite pmid|25803711 }}</ref> Multimodal holographic microscopy: distinction between apoptosis and oncosis #MMPMID25803711 Balvan J ; Krizova A ; Gumulec J ; Raudenska M ; Sladek Z ; Sedlackova M ; Babula P ; Sztalmachova M ; Kizek R ; Chmelik R ; Masarik M PLoS One 2015[]; 10 (3 ): e0121674 PMID25803711 show ga Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI- phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis. |*Apoptosis [MESH] |Cell Line, Tumor [MESH] |Cell Survival [MESH] |Holography/*methods [MESH] |Humans [MESH] |Microscopy, Fluorescence/*methods [MESH] |Multimodal Imaging/*methods [MESH] |Necrosis [MESH] |Phenotype [MESH]Multimodal holographic microscopy: distinction between apoptosis and o(epmc).pdf DeepDyve Pubget Overpricing