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10.1002/0471142905.hg1507s83 http://scihub22266oqcxt.onion/10.1002/0471142905.hg1507s83 C4519007!4519007 !25271839     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25271839 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Curr+Protoc+Hum+Genet 2014 ; 83 (ä): 15.7.1-27 Nephropedia Template TP {{tp|p=25271839 |t=2014. Mouse Genome Editing Using the CRISPR/Cas System |pdf=|usr=}}{{25271839 }} gab.com Text Mouse Genome Editing Using the CRISPR/Cas System JO: Curr Protoc Hum Genet http://www.kidney.de/pget.php?&tw=1&pmid=25271839 #FOAvip The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one-cell mouse embryos to create knockout or knock-in mouse models. Twit Text FOAVip Mouse Genome Editing Using the CRISPR/Cas System ????Curr Protoc Hum Genet http://www.kidney.de/pget.php?&tw=1&pmid=25271839 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25271839 #FOAvip English Wikipedia <ref>{{Cite pmid|25271839 }}</ref> Mouse Genome Editing Using the CRISPR/Cas System #MMPMID25271839 Harms DW ; Quadros RM ; Seruggia D ; Ohtsuka M ; Takahashi G ; Montoliu L ; Gurumurthy CB Curr Protoc Hum Genet 2014[Oct]; 83 (ä): 15.7.1-27 PMID25271839 show ga The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one-cell mouse embryos to create knockout or knock-in mouse models. |*Clustered Regularly Interspaced Short Palindromic Repeats [MESH] |*Genome [MESH] |Animals [MESH]Mouse Genome Editing Using the CRISPR/Cas System (epmc).pdf DeepDyve Pubget Overpricing