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2017 ; 18
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Monitoring and Measuring Autophagy
#MMPMID28846632
Yoshii SR
; Mizushima N
Int J Mol Sci
2017[Aug]; 18
(9
): ä PMID28846632
show ga
Autophagy is a cytoplasmic degradation system, which is important for starvation
adaptation and cellular quality control. Recent advances in understanding
autophagy highlight its importance under physiological and pathological
conditions. However, methods for monitoring autophagic activity are complicated
and the results are sometimes misinterpreted. Here, we review the methods used to
identify autophagic structures, and to measure autophagic flux in cultured cells
and animals. We will also describe the existing autophagy reporter mice that are
useful for autophagy studies and drug testing. Lastly, we will consider the
attempts to monitor autophagy in samples derived from humans.