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10.1007/978-1-4939-6393-5_14 http://scihub22266oqcxt.onion/10.1007/978-1-4939-6393-5_14 C5373554!5373554 !27590159     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27590159 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2016 ; 1481 (ä): 127-40 Nephropedia Template TP {{tp|p=27590159 |t=2016. Monitoring Wnt/?-Catenin Signaling in Skin |pdf=|usr=}}{{27590159 }} gab.com Text Monitoring Wnt/ -Catenin Signaling in Skin JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27590159 #FOAvip Wnt signaling through ?-catenin plays a crucial role in skin development and homeostasis. Disruption or hyperactivation of this pathway results in skin defects and diseases (Lim and Nusse, Cold Spring Harb Perspect Biol 5(2), 2013). Monitoring Wnt signaling in skin under normal and abnormal conditions is therefore critical to understand the role of this pathway in development and homeostasis.In this chapter, we provide methods to detect Wnt/?-catenin (canonical) signaling in the skin. We present a comprehensive list of Wnt reporter mice and detail the processing of skin tissue to detect reporter genes. From this list, we focus on the three most recent lines that, according to reports, are the most sensitive in skin. Additionally, we describe a protocol to detect nuclear ?-catenin, a hallmark of active Wnt signaling, although this technique should be used with caution due to its limited sensitivity. The techniques outlined below will be useful for detecting active Wnt signaling in skin. Twit Text FOAVip Monitoring Wnt/ -Catenin Signaling in Skin ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27590159 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27590159 #FOAvip English Wikipedia <ref>{{Cite pmid|27590159 }}</ref> Monitoring Wnt/?-Catenin Signaling in Skin #MMPMID27590159 Ku AT ; Miao Q ; Nguyen H Methods Mol Biol 2016[]; 1481 (ä): 127-40 PMID27590159 show ga Wnt signaling through ?-catenin plays a crucial role in skin development and homeostasis. Disruption or hyperactivation of this pathway results in skin defects and diseases (Lim and Nusse, Cold Spring Harb Perspect Biol 5(2), 2013). Monitoring Wnt signaling in skin under normal and abnormal conditions is therefore critical to understand the role of this pathway in development and homeostasis.In this chapter, we provide methods to detect Wnt/?-catenin (canonical) signaling in the skin. We present a comprehensive list of Wnt reporter mice and detail the processing of skin tissue to detect reporter genes. From this list, we focus on the three most recent lines that, according to reports, are the most sensitive in skin. Additionally, we describe a protocol to detect nuclear ?-catenin, a hallmark of active Wnt signaling, although this technique should be used with caution due to its limited sensitivity. The techniques outlined below will be useful for detecting active Wnt signaling in skin. |Animals [MESH] |Homeostasis [MESH] |Mice [MESH] |Molecular Biology/*methods [MESH] |Skin/growth & development/*metabolism [MESH] |Wnt Signaling Pathway/*genetics [MESH]Monitoring Wnt/?-Catenin Signaling in Skin (epmc).pdf DeepDyve Pubget Overpricing