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10.1165/rcmb.2015-0212OC http://scihub22266oqcxt.onion/10.1165/rcmb.2015-0212OC C4942207!4942207 !26807608     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26807608 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Respir+Cell+Mol+Biol 2016 ; 55 (1 ): 128-34 Nephropedia Template TP {{tp|p=26807608 |t=2016. Molecular Analysis of Sarcoidosis Granulomas Reveals Antimicrobial Targets |pdf=|usr=}}{{26807608 }} gab.com Text Molecular Analysis of Sarcoidosis Granulomas Reveals Antimicrobial Targets JO: Am J Respir Cell Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=26807608 #FOAvip Sarcoidosis is a granulomatous disease of unknown cause. Prior molecular and immunologic studies have confirmed the presence of mycobacterial virulence factors, such as catalase peroxidase and superoxide dismutase A, within sarcoidosis granulomas. Molecular analysis of granulomas can identify targets of known antibiotics classes. Currently, major antibiotics are directed against DNA synthesis, protein synthesis, and cell wall formation. We conducted molecular analysis of 40 sarcoidosis diagnostic specimens and compared them with 33 disease control specimens for the presence of mycobacterial genes that encode antibiotic targets. We assessed for genes involved in DNA synthesis (DNA gyrase A [gyrA] and DNA gyrase B), protein synthesis (RNA polymerase subunit ?), cell wall synthesis (embCAB operon and enoyl reductase), and catalase peroxidase. Immunohistochemical analysis was conducted to investigate the locale of mycobacterial genes such as gyrA within 12 sarcoidosis specimens and 12 disease controls. Mycobacterial DNA was detected in 33 of 39 sarcoidosis specimens by quantitative real-time polymerase chain reaction compared with 2 of 30 disease control specimens (P?<?0.001, two-tailed Fisher's test). Twenty of 39 were positive for three or more mycobacterial genes, compared with 1 of 30 control specimens (P?<?0.001, two-tailed Fisher's test). Immunohistochemistry analysis localized mycobacterial gyrA nucleic acids to sites of granuloma formation in 9 of 12 sarcoidosis specimens compared with 1 of 12 disease controls (P?<?0.01). Microbial genes encoding enzymes that can be targeted by currently available antimycobacterial antibiotics are present in sarcoidosis specimens and localize to sites of granulomatous inflammation. Use of antimicrobials directed against target enzymes may be an innovative treatment alternative. Twit Text FOAVip Molecular Analysis of Sarcoidosis Granulomas Reveals Antimicrobial Targets ????Am J Respir Cell Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=26807608 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26807608 #FOAvip English Wikipedia <ref>{{Cite pmid|26807608 }}</ref> Molecular Analysis of Sarcoidosis Granulomas Reveals Antimicrobial Targets #MMPMID26807608 Rotsinger JE ; Celada LJ ; Polosukhin VV ; Atkinson JB ; Drake WP Am J Respir Cell Mol Biol 2016[Jul]; 55 (1 ): 128-34 PMID26807608 show ga Sarcoidosis is a granulomatous disease of unknown cause. Prior molecular and immunologic studies have confirmed the presence of mycobacterial virulence factors, such as catalase peroxidase and superoxide dismutase A, within sarcoidosis granulomas. Molecular analysis of granulomas can identify targets of known antibiotics classes. Currently, major antibiotics are directed against DNA synthesis, protein synthesis, and cell wall formation. We conducted molecular analysis of 40 sarcoidosis diagnostic specimens and compared them with 33 disease control specimens for the presence of mycobacterial genes that encode antibiotic targets. We assessed for genes involved in DNA synthesis (DNA gyrase A [gyrA] and DNA gyrase B), protein synthesis (RNA polymerase subunit ?), cell wall synthesis (embCAB operon and enoyl reductase), and catalase peroxidase. Immunohistochemical analysis was conducted to investigate the locale of mycobacterial genes such as gyrA within 12 sarcoidosis specimens and 12 disease controls. Mycobacterial DNA was detected in 33 of 39 sarcoidosis specimens by quantitative real-time polymerase chain reaction compared with 2 of 30 disease control specimens (P? |*Molecular Targeted Therapy [MESH] |Adolescent [MESH] |Adult [MESH] |Aged [MESH] |Anti-Infective Agents/*therapeutic use [MESH] |Case-Control Studies [MESH] |DNA, Bacterial/analysis [MESH] |Demography [MESH] |Female [MESH] |Granuloma/*drug therapy/microbiology/pathology [MESH] |Humans [MESH] |In Situ Hybridization [MESH] |Male [MESH] |Middle Aged [MESH] |Mycobacterium/drug effects [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Sarcoidosis/*drug therapy/microbiology/pathology [MESH]Molecular Analysis of Sarcoidosis Granulomas Reveals Antimicrobial Tar(epmc).pdf DeepDyve Pubget Overpricing