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2016 ; 67
(7
): 2093-105
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Modulation of R-gene expression across environments
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MacQueen A
; Bergelson J
J Exp Bot
2016[Mar]; 67
(7
): 2093-105
PMID26983577
show ga
Some environments are more conducive to pathogen growth than others, and, as a
consequence, plants might be expected to invest more in resistance when pathogen
growth is favored. Resistance (R-) genes in Arabidopsis thaliana have unusually
extensive variation in basal expression when comparing the same R-gene among
accessions collected from different environments. R-gene expression variation was
characterized to explore whether R-gene expression is up-regulated in
environments favoring pathogen proliferation and down-regulated when risks of
infection are low; down-regulation would follow if costs of R-gene expression
negatively impact plant fitness in the absence of disease. Quantitative reverse
transcription-PCR was used to quantify the expression of 13 R-gene loci in plants
grown in eight environmental conditions for each of 12 A. thaliana accessions,
and large effects of the environment on R-gene expression were found.
Surprisingly, almost every change in the environment--be it a change in biotic or
abiotic conditions--led to an increase in R-gene expression, a response that was
distinct from the average transcriptome response and from that of other stress
response genes. These changes in expression are functional in that environmental
change prior to infection affected levels of specific disease resistance to
isolates of Pseudomonas syringae. In addition, there are strong latitudinal
clines in basal R-gene expression and clines in R-gene expression plasticity
correlated with drought and high temperatures. These results suggest that
variation in R-gene expression across environments may be shaped by natural
selection to reduce fitness costs of R-gene expression in permissive or
predictable environments.